Phylogenetic Relationships of Opsopoeodus emiliae, with Comments on the Taxonomic Implications of Discordance among Datasets
Mitochondrial (cyt b and ND2) and nuclear gene (S7 intron 1) sequences were generated to evaluate phylogenetic relationships and validity of the monotypic minnow genus Opsopoeodus. Parsimony and Bayesian phylogenetic analyses of the mitochondrial markers resulted in largely unresolved relationships among ingroup taxa. Relationships recovered from the nuclear intron were well resolved and consistent with previously published morphological, chromosomal, and behavioral data that supported the recognition of Opsopoeodus as a genus. Although mitochondrial genes recovered species as strongly supported clades, they provided limited resolution of relationships among ingroup species and genera and showed high levels of saturation at inter-genus levels of divergence. These findings re-emphasize the importance of considering multiple datasets in making taxonomic decisions and caution against use of single gene trees alone.
Majority rule consensus of 75,000 post-burn-in trees resulting from the Bayesian analyses of the mitochondrial cytochrome b gene. Numbers above nodes are posterior probability values followed by bootstrap support values. Letters after species names correspond to letters provided in Table 1.
Majority rule consensus of 75,000 post-burn-in trees resulting from the Bayesian analyses of the partial mitochondrial ND2 gene. Numbers on nodes are posterior probability values followed by bootstrap support values. Letters after species names correspond to letters provided in Table 1.
Majority rule consensus of 75,000 post-burn-in trees resulting from the Bayesian analyses of S7 intron 1. Numbers on nodes are posterior probability values followed by bootstrap support values. For nodes with three numbers, numbers are posterior probability values followed by bootstrap values when gaps were excluded and bootstrap values with gaps included as a fifth character state. Letters after species names correspond to letters provided in Table 1.
Majority rule consensus of 75,000 post-burn-in trees resulting from the Bayesian analyses of the mitochondrial cytochrome b gene and the nuclear S7 intron 1. Numbers above nodes are posterior probability values. Letters after species names correspond to letters provided in Table 1.
Contributor Notes
Associate Editor: D. Buth.