Reproductive Ecology of Nest Associates: Use of RFLPs to Identify Cyprinid Eggs
In this study, we use restriction fragment length polymorphisms (RFLP) of the mtDNA ND2 gene to identify to species cyprinid eggs collected from a spawning aggregation substrate in the Pascagoula River system. A library was generated with inter- and intra-specific RFLP patterns for nine cyprinids: Notropis baileyi, N. texanus, N. longirostris, N. amplamala, Nocomis leptocephalus, Luxilus chrysocephalus, Lythrurus roseipinnis, and Cyprinella venusta. Eggs of three species were identified from a single nest, while only one species was observed spawning at the site. These results confirm that RFLP is an effective method for identifying unknown eggs of nest associates, and North American cyprinids in general.Abstract
RFLP banding patterns for all nine focal taxa. The most common patterns for species with variation are represented here. Lane 1 is a non-cut ND2 amplification used as a control marker; Lanes 4, 8, and 11 are NEB 100 bp ladders; Lane 2 is Notropis baileyi; Lane 3 is Nocomis leptocephalus; Lane 5 is Notropis longirostris; Lane 6 is Notropis texanus; Lane 7 is Luxilus chrysocephalus; Lane 9 is Cyprinella venusta; Lane 10 is Lythrurus roseipinnis; Lane 12 is Hybopsis winchelli; and Lane 13 is Notropis amplamala.
Contributor Notes
Associate Editor: W. L. Smith.