Editorial Type:
Article Category: Research Article
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Online Publication Date: 01 Aug 2004

Methods for Preparing Dry, Partially Articulated Skeletons of Osteichthyans, with Notes on Making Ridewood Dissections of the Cranial Skeleton

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Page Range: 603 – 609
DOI: 10.1643/CI-03-054R1
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Abstract

We describe methods for preparing dry skeletons of virtually any osteichthyan species with a well-ossified skeleton, including very large specimens (e.g., > 1 m Megalops atlanticus). Our approach differs from those conventionally used to prepare skeletons of tetrapods in that (1) fairly complete dissection of the specimen is required at the outset of processing; and (2) we use an alcohol dehydration step to rapidly dry the specimen. Similar techniques can be used to prepare well-calcified chondrichthyan skeletons. We also outline the steps for making Ridewood dissections of the skull. Dry, partially articulated skeletons prepared by these methods can be stored indefinitely in acid-free containers in an environmentally controlled space (21 C ± 3 C; Rh = 40% ± 5%) in pest-proof specimen cases. Although a truism of anatomical research is that you cannot learn everything from studying one specimen or one type of preparation, partially articulated dry skeletons are useful for research ranging from phylogenetic investigations to age and growth analyses to functional morphology, making them of great and lasting value to any collection.

Copyright: The American Society of Ichthyologists and Herpetologists
 Fig. 1. 
 Fig. 1. 

A dry, partially articulated skeleton of an adult ladyfish, Elops saurus prepared using the Ridewood dissection method, in which the gill arches (A) and right side of the head (B) were dissected away from the rest of the skull (C). To ensure thorough cleaning, the paired (D, E) and median fins (F, G) were isolated, and the vertebral column was separated into abdominal (H) and caudal (I) regions. Scale bar = 5 cm


 Fig. 2. 
 Fig. 2. 

A partially articulated specimen of a large Atlantic tarpon, Megalops atlanticus, in a suitable box ready for the dermestid colony. Each piece has a tag with specimen's field number. This specimen was prepared using the Ridewood dissection method; the inner surface of the right cheek and jaws is visible below and the gill arches can be seen on the left


Contributor Notes

(WEB, AMR, CDL) Department of Biology, University of Massachusetts, 611 North Pleasant Street, Amherst, Massachusetts 01003–0027; (EJH, LG) Geology Department, Field Museum of Natural History, 1400 South Lake Shore Drive, Chicago, Illinois 60605; (BB, RA) Department of Ichthyology, American Museum of Natural History, Central Park West at 79th Street, New York, New York 10024; (PLF) Department of Palaeontology, Natural History Museum, Cromwell Road, London, U.K., SW7 5BD; and (GJN)School of Botany, University of Melbourne, Parkville, Victoria 3052, Australia. (WEB) wbemis@bio.umass.edu; (AMR) alanr@bio.umass.edu; (CDL) clittle@bio.umass.edu; (EJH) ehilton@fieldmuseum.org; (LG) grande@fmnh.org; (BB) bbrown@amnh.org; (RA) arrin@amnh.org; (PLF) p.forey@nhm.ac.uk; and (GJN) g.nelson@botany.unimelb.edu.au

Received: 06 Mar 2003
Accepted: 01 May 2004
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