Phylogenetic Relationships of the Hybopsis amblops Species Group (Teleostei: Cyprinidae)
We examined the phylogenetic relationships among members of the Hybopsis amblops species group in a total evidence cladistic analysis using DNA sequence data from regions of three mitochondrial genes and previously published morphological data. Molecular characters consisted of 120 informative sites from 811 bases of the 12S, 82 informative sites from 571 bases of the 16S gene, and 214 informative sites from 519 bases from the ND2 protein-coding gene. Morphological characters consisted of 14 transformation series previously published by R. L. Mayden in 1989. Two most parsimonious trees were found with the topologies: (Hybopsis hypsinotus ((Hybopsis lineapunctata, Hybopsis rubrifrons) (Hybopsis amblops (Hybopsis amnis (Hybopsis winchelli, Hybopsis sp.))))) and (Hybopsis hypsinotus (Hybopsis amblops ((Hybopsis lineapunctata, Hybopsis rubrifrons) (Hybopsis amnis (Hybopsis winchelli, Hybopsis sp.))))).Abstract
Most-parsimony trees resulting from 500 heuristic replications. Two topologies were found (TL = 831, CI = 0.619, HI = 0.381, RI = 0.787, RC = 0.487). Numbers to the left of the internodes represent molecular TS with a c.i. of ≥ 0.5. Numbers to the right of the internodes represent molecular TS with a c.i. of < 0.5, followed by the number of morphological TS supporting the node. (A) First of two most-parsimonious resolutions. The node uniting Hybopsis amblops and the Gulf Coast clade (indicated by the solid bar) consisted of 11 characters (one morphological) under ACCTRAN optimization. (B) Second of two most-parsimonious resolutions. The node uniting H. amblops and the rest of the species group (indicated by the solid bar) consisted of 42 characters (two morphological) under ACCTRAN optimization. (C) Consensus tree of two most-parsimonious trees with Bremer decay indices (left) and bootstrap proportions for 10,000 replicates (right) reported at each node